formalin-fixed, paraffin-embedded normal human kidney sections  (Novus Biologicals)

Journal: Cancer Science

Article Title: Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10

doi: 10.1111/j.1349-7006.2007.00701.x

Figure Lengend Snippet: Immunohistochemical staining analyses of synovial sarcoma and normal human tissue sections using MAb 92‐13; (a,b) synovial sarcoma tissue sections (c) colon, (d) heart, (e) lung, (f) liver, (g) kidney and (h) placenta. Original magnification ×100. Bar, 50 µm.

Article Snippet: For immunohistochemical staining analysis, 4‐µm sections of paraffin‐embedded normal adult human tissues (lung, heart, liver, kidney, colon and placenta) (BioChain, Hayward, CA, USA) and surgical synovial sarcoma specimens were deparaffinized and processed for antigen retrieval in Target Retrieval Solution (pH 9) (DAKO Cytomation, Carpinteria, CA, USA) at 125°C for 30 s. After treatment with peroxidase blocking reagent (DAKO Cytomation) for 10 min, followed by treatment with protein blocking serum‐free (DAKO Cytomation) for 30 min, slides were incubated with 10 µg/mL MAb 92‐13.

Techniques: Immunohistochemical staining, Staining